Identification of a natural mangrove hybrid of Rhizophora in Thailand using SNPs in nuclear and chloroplast regions Suchitra Changtragoon1 1Forest and Plant Conservation Research office, Department of National Parks, Wildlife and Plant Conservation, Bangkok 10900, Email: suchitra.changtragoon@gmail.com Sonjai Havanond2 2Sirindhorn International Environmental Park, Phetchaburi, Thailand Email: sonjai_h@hotmail.com Kittiya Singthong1 Email: singthong.k@gmail.com Abstract One vigorously established 9m tall putative hybrid of Rhizophora mucronata and R. apiculata was found in the regenerating mangrove forest within the Sirindhorn International Environmental Park (SIEP) at Phetchaburi Thailand. To confirm the hybrid status of this tree, nucleotide variation was examined at three nuclear regions together with one chloroplast region. The nuclear regions were DLDH, SBE2 and FMRrmll; atpB-rbcL spacer was the chloroplast region. All were PCR amplified and sequenced. The single nucleotide polymorphisms (SNPs) in the three nuclear regions demonstrated nucleotide variation specific to R. mucronata and R. apiculata. The hybrid displayed the same nucleoptide polymorphisms as those from both R. mucronta and R. apiculata, thus revealing that this hybrid is a natural hybrid of R. mucronta and R. apiculata. The single nucleotide polymorphisms in atpB-rbcL chloroplast region in the hybrid were identical to those of R. mucronta, showing that the mother tree of this hybrid was R. mucronta. This finding may present useful genetic resources for mangrove reforestation in more challenging habitats where hybrid vigour may be advantageous. Keywords: natural hybrid, Rhizorphora mucronata x R. apiculata, single nucleotide polymorphisms (SNP’s) novel hybrid vigour.
Programme & Abstract for the Presentation : International Mangrove Forum Mangrove Management Towards Sustainable Development Goals (SDGs) 23 August 2018 @ 13.00-17.00 hrs. FORTROP Room, 3rd Floor, 60th Year Building Faculty of Forestry, Kasetsart University, Bangkok
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