Table 2 Micropropagation of Acacia nilotica subsp. indica, Allium stracheyi and Naravelia zeylanica Acacia nilotica sub sp. indica* Allium stracheyi Naravelia zeylanica Explant Leaf Leaf, Petiole Leaf, Internodes Callogenesis IAA 1.0 + IBA 1.0 IAA 1.0 + 2,4-D 1.5 2,4 –D 2.5+ BAP 0.5 Somatic IAA 4.0 IBA 5.0 2,4-D 3.0 embryogeneis Organogeneis – Shoot BAP 2.0+ IAA 0.5 BAP 2.0 + IBA 1.0 BAP 2.0+ IAA 0.5 Organogeneis – Root IAA 0.5 IAA 0.5 + NAA 0.5 ½ MS + IAA 0.5 * WPM is used callus color, texture of calli, width and weight were observed. Somatic embryogenesis time taken, number of embryos emerged, in case of organogenesis time taken, number of shoot, roots formed and its length were also recorded (data not shown). In vitro micropropagation via callogenesiss, induction of somatic embryogenesis and organogenesis is essential for plant genetic resource management and has become increasingly important for conservation of rare plant species (Iankova et al. 2001; Bhatia et al. 2002). Unfortunately, no serious attempts have been made to conserve this immense treasure of traditional knowledge of ethanobotincals associated with the indigenous people (Siva, et al. 2009). This is the first attempt to find the micropropagation protocol for Acacia nilotica subsp. indica, Allium stracheyi and Naravelia zeylanica. Table 3 Yield potentials of leaf extracts of Acacia nilotica subsp. indica, Allium stracheyi and Naravelia zeylanica Extract % Yield Acacia nilotica sub sp. indica Allium stracheyi Naravelia zeylanica PE 19.82 14.94 15.08 BEN 16.63 18.28 - BUT - 16.53 - DCM 3.00 - - CF 3.61 - 23.60 EAC - 2.66 6.20 ETH 35.07 11.56 15.41 ACT - - 9.01 AQ 15.63 29.54 20.22 PE: Petroleum ether; BEN: Benzene; BUT: Butanol; CF: Chloroform; DCM: Dichloromethane; EAC: Ethyl acetate; ETH: Ethanol; ACT: Acetone; AQ: Water Proceedings of the International Conference on Climate Change, Biodiversity and Ecosystem Services for the 111 Sustainable Development Goals (SDGs): Policy and Practice 27-29 June 2016, Cha-am, Phetchaburi, Thailand
Proceedings of International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals : Policy and Practice 27-29 June 2016 at the Sirindhorn International Environmental Park, Cha-am, Phetchaburi, Thailand
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