Fig.6 Pictures of Rhizophora mucronata putative hybrid of Rhizophora and Rhizophora apiculata Fig.5 Putative hybrid PCR reaction profile comprised an initial denaturation of 3 min at 95°C; followed by 35 cycles of 30 s at 95°C, 30 s at Ta, and 2 min at 72°C; and finally an extension step at 72°C for 7 min. Purified PCR products were used for direct sequencing. Sequencing reactions were carried out using the BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and analyzed on an ABI 3730 DNA Analyzer (Applied Biosystems). Data analyses Nucleotide sequences were assembled and edited using the software BioEdit version 7.2 Sequences of each locus were then aligned using ClustalW (Thompson et. al. 1997). III. RESULTS The final aligned lengths of the nDNA loci were 1221 bp for DLDH, 947 bp for SBE2 and 609 bp for FMRrm11. Species- specific sites were observed at all the loci. The putative natural hybrid individual from SIEP Table 1 PCR primers used in this study had the same single nucleotide polymorphisms as from both R. mucronata and R. apiculata (Figure 7). This reveals that the putative natural hybrid is a natural hybrid of R. mucronata and R. apiculata. As the chloroplast genome is inherited uniparetally, to identify which Rhizophora species would be the mother tree of the natural hybrid, the DNA sequence of the cholorplast gene (atpB-rbcL intergenic spacer) from R. apiculata, R. mucronata and the putative hybrid was compared. The natural hybrid individual from SIEP had the same single nucleotide polymorphisms as R. mucronata. As shown in Figure 8 the results indicate that R. mucronata is the mother tree of this natural hybrid. Proceedings of the International Conference on Climate Change, Biodiversity and Ecosystem Services for the 55 Sustainable Development Goals (SDGs): Policy and Practice 27-29 June 2016, Cha-am, Phetchaburi, Thailand
Proceedings of International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals : Policy and Practice 27-29 June 2016 at the Sirindhorn International Environmental Park, Cha-am, Phetchaburi, Thailand
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