differentiation as expected in outcrossing species. The high level of genetic polymorphism and genetic differentiation revealed by RAPD analysis might play a role in the dynamic evolution of Gloriosa in south India. V. CONCLUSION The results of this study indicated that RAPD are sufficiently informative and powerful to assess genetic variability in Gloriosa superba. The estimate ofgenetic variation reported herein provides a basis for the in situ conservation and exploitation of genetics resources in this species. From a conservation point of view population 7 (Mandya) and population 9 (Kozhikode) deserve special consideration as the former displayed elite population due to the high level of alkaloid content (colchicine) and the later expressed high genetic diversity there by denoting maximum phylogenetic adaptation. The present study assumes significance as it provides valuable information on the nature and pattern of genetic variation existing in this medicinally important species. Evaluation of Gloriosa superba germplasm showed a large variation in the quantitative traits between populations. The population from Mandya was considered as the elite population of Gloriosa superba as a result of linking the phenotypic variability with the chemical relatedness of population the cluster based on the important alkaloid colchicine. REFERENCES 1 Gupta LM, Rana RC, Raina R, Meenakshi G (2005). Colchicine content in Gloriosa superba L. SKUAST-J J Res 4: 238-241. 2 Yadav K, Aggarwal A, Singh N (2012). Actions for ex situ conservation of Gloriosa superba L. - an endangered ornamental cum medicinal plant. J Crop Sci Biot 15: 297-303. 3 Prasad MNV, Padmalatha K, Jayaram K, Raju NL, Teixeira DA, Silva JA (2007). Medicinal plants from Deccan ecoregion, India: Traditional knowledge, ethnopharmacology, cultivation, utilization, biotechnology and conservation-Opportunities and impediments. Med. Arom. Plants SciBiot 1: 155-208. 4 Deborah YQH, Lau AJ, Yeo CL, Liu XK, Yang CR, Koh HL, et. al. Geneticdiversity and variation of saponin contents in Panaxnotoginseng rootsfrom a single farm. J Agri Food Chem 2005; 53: 8460-7. 5 Falconer DS. Introduction to quantitative genetics. Edinbourgh/London: Oliver & Boyd; 1964. 6 Klyushnichenko VE, Yakimov SA, Tuzova TP, SyagailoYa V, Kuzovkina IN, Wulfson AN, et. al. Determination of indole alkaloids from R. chromatography 1995; 704: 357-62. 7 Maroof SMA, Soliman K, Jorgensen RA, Allard RW. Ribosomal DNA spacer length p o lymo r p h isms in bar ley : Mendel ian inheritancechromodomal location and population dynamics. Proc Nat AcadSci 1984; 81: 8014-8. 8 Sambrook J, Frisch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbour Press; 1989. 9 Yeh FC, Yang RC, Boyle T. POPGENE 32-version 1.31. Population Genetics Software. http.//www.ualberta.ca/~fyeh/fyeh/; 1999. 10 Nei M. Analysis of gene diversity in subdivided populations. Proc NatlAcadSci USA 1973; 70: 3321-3. 11 Lewinton RC. The apportionment of 68 Proceedings of the International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals (SDGs): Policy and Practice 27-29 June 2016, Cha-am, Phetchaburi, Thailand
Proceedings of International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals : Policy and Practice 27-29 June 2016 at the Sirindhorn International Environmental Park, Cha-am, Phetchaburi, Thailand
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