much as all parts of the plant having medicinal value. In nature, less seed germination with poor viability is responsible for its diminishing population size. The poor propagation coupled with over-exploitation companies has put this plant into acutely threatened status for the local population as well as a pharmaceutical species 2. Therefore, tracing successfully adapted variants at genetic level of G. superba is of immediate necessity for the long-term preservation of the species. For efficient conservation and management, the genetic composition of the species in different geographic locations needs to be assessed 3. There are also studies on correlation between genetic diversity and variation in chemical constituents 4. Quantitative and qualitative status of active constituents along with genetic diversity in a medicinal plant would help to devise conservation strategies and selection of right sample for maximum yield. Therefore, knowledge of genetic diversity and s tructure among Gloriosa superba populations is required for the development of appropriate conservation and breeding programs. Thus, we address the f o l lowing questions: (i) do chemical and molecular markers provide similar conclusions about population differentiation; (ii) is there a relationship between chemical and RAPD data and (iii) what is the implication of the detected genetic variation for conservation strategies? Traditionally, genetic diversity is determined through the analyses of morphological parameters. This method includes serious l imi t a t ions s u ch a s the i n f luenc e of environmental conditions, giving results that represent just a part of total genetic diversity 5. II. MATERIALS AND METHODS The plant materials were collected from various parts of south India (Fig.1) during August 2015 to March 2016. A total of 10 populations of Gloriosa superba were used in this study. A complete list of populations and their sources are available in Fig.1 and Table 1. Fig.1 Location of natural populations of Gloriosa superba collected during the study Proceedings of the International Conference on Climate Change, Biodiversity and Ecosystem Services for the 59 Sustainable Development Goals (SDGs): Policy and Practice 27-29 June 2016, Cha-am, Phetchaburi, Thailand
Proceedings of International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals : Policy and Practice 27-29 June 2016 at the Sirindhorn International Environmental Park, Cha-am, Phetchaburi, Thailand
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