Propagules of collected plants (each population) were raised from root cuttings and plants were grown in the greenhouse of Botanical Garden, Department of Botany, Annamalai University, Annamalai Nagar, Tamil Nadu. Stem cuttings were collected from 15 Table 1 Populations and collection details of Gloriosa superba from the study area The plant samples were collected from ten populations of the d i ff e r e n t geographical positions of South India. Such as Udaiyarpalaiyam, Yelagiri Hills, Pallakkodu, Kalrayan Hills, Nagercoil, Bhavani, Mandya, Bangalore, Kozhikode and Kottayam. Morphological characterization Morphological features utilized for characterization were plant height, number of branches/plant, leaf length, leaf width, number of flowers per cyme, number of fruits per cyme, tuber length and tuber yield. Assessment of alkaloid content of various populations of Gloriosa superba Total alkaloid contents were estimated from tubers of Gloriosa superb collected from different habitats. 100 g of powdered dry samples individuals of each population. Individuals were chosen at random and young shoots were sprouted from the cuttings after one month of planting. From each population, 10 individuals each were selected for molecular genetic analyses. of Gloriosa superb was soaked in 200 mL methanol and left for 30 min. After 30 min, the soaked plant material was filtered. The residue obtained after filtration is further dissolved in 5 mL methanol and filtered. After 10 mins, the same step is repeated once again and the final filtrate is collected in 50 mL conical flask. The extract was evaporated to dryness in the Soxhlet. evaporator. The crude extract was dissolved in 100 mL of 0.01 M HCl. The pH of filtered solutions was adjusted to 6.0 with 0.01 M NaOH. The crude extracts obtained were used for HPLC analysis 6. The crude extract obtained was concentrated to dryness to yield crude alkaloid fraction (CAF). The extract of each plant was transferred to 5 mL volumetric flasks separately with the help of methanol (HPL Cgrade), sonicated for a few minutes and filtered and the volume was made up to mark with methanol; 0.004% of Colchicine in methanol was used as standard. 60 Proceedings of the International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals (SDGs): Policy and Practice 27-29 June 2016, Cha-am, Phetchaburi, Thailand
Proceedings of International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals : Policy and Practice 27-29 June 2016 at the Sirindhorn International Environmental Park, Cha-am, Phetchaburi, Thailand
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