Population genetic structure and level of genetic diversity in Gloriosa superba After screening 30 primers 18 primers produced polymorphic and repeatable products (Table 3). The banding profile and polymorphism generated are shown in Plate 2. The total number of amplified DNA band varied between 21 (Primer OPC-05) and 8 (primer OPW-02) with the average of 13 band per primer. The maximum number of polymorphic bands (21) was obtained with primer OPC-05. The polymorphism percentage ranges from 81.25% (OPA-03) to as high as 100% for 3 primers (Primer OPC-13, OPP-08, and OPP-05). Average polymorphism across population was found to be 91.94% (Table 3). Overall size of the PCR amplified product band ranged from 319 bp to 2260 bp (Table 3). The intraspecific level of RAPD polymorphism was measured as the proportions of polymorphic loci to the total number of loci scored in all accessions of the same species. The average gene diversity (HT) over all loci was 0.4265, while for individual loci it ranged from 0.2344 (Yelagiri Hills,) to 0.2968 (Kozhikode). Table 3 The nucleotide sequence of the selected primers and the numbers of amplified and polymorphic bands scored ** Those not replicated. 64 Proceedings of the International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals (SDGs): Policy and Practice 27-29 June 2016, Cha-am, Phetchaburi, Thailand
Proceedings of International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals : Policy and Practice 27-29 June 2016 at the Sirindhorn International Environmental Park, Cha-am, Phetchaburi, Thailand
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