Plate 2 RAPD-PCR ampliflication of genomic DNA from Gloriosa superba M: marker; Lanes 1-10: Populations 1-10 The average expected heterozygosity (Hs) was 1.7657 with a range from 1.4121 to 1.5236. across all loci in the total sample was 0.2600. Populations from Kozhikode (population 9) had The average coefficient of gene differentiation the largest value of Ne and h while the (GST) over total loci was 0.3903 (P 0.01). The population from Udaiyarpalaiyam (population observed number of alleles (Na) per population 1) had the lowest (Table 4). Using Slatkin and varied from 1.5784 to 1.7459 (Table 4) and for Barton 16 formula for estimated gene flow all populations is 2.000. The mean effective (Nm) = 0.25 (1-GST)/GST the gene flow number of alleles (Ne) 15 for all populations between all population was 0.7811. Table 4 Analysis of genetic polymorphism obtained with RAPD primers in various populations of Gloriosa superba Ne-Effective number of alleles, h-Nei’s gene diversity, I-Shannon’s Information index, NPL- The number of polymorphic loci, PPL-The percentage of polymorphic loci Proceedings of the International Conference on Climate Change, Biodiversity and Ecosystem Services for the 65 Sustainable Development Goals (SDGs): Policy and Practice 27-29 June 2016, Cha-am, Phetchaburi, Thailand
Proceedings of International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals : Policy and Practice 27-29 June 2016 at the Sirindhorn International Environmental Park, Cha-am, Phetchaburi, Thailand
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